Andor Multimodal Fast Confocal Microscope System Dragonfly

Dragonfly

Andor

・Spinning disk confocal imaging with uniform Borealis illumination
・Fast live-cell imaging up to 400 fps and 3D imaging for large volume samples
・4 laser lines (405, 488, 561, 637 nm)
・2 high-sensitive cameras (iXon Ultra 888 EMCCD and Zyla 4.2 Plus sCMOS)
・Mosaic3 photostimulation unit with 405 nm laser
・Dragonfly 502
Spinning disk with 2 pinhole diameters (25 and 40 microns), Borealis illumination, Laser-illuminated widefield mode, 2 camera ports, Camera magnification (3-position motorized), Illumination zoom, GPU-accelerated deconvolution in parallel with acquisition, Imaris Core
・Motorized inverted microscope Nikon Ti2-E
・Lasers 405 nm/100 mW, 488 nm/150 mW, 561 nm/100 mW, 637 nm/140 mW
・Cameras
iXon Ultra EMCCD
Zyla 4.2 Plus sCMOS　
・Automatic focus correction unit (Perfect Focus)
・ASI motorized XY stage and stage piezo (500-micron range)
・Objectives
CFI Plan Apochromat Lambda 10X Dry NA 0.45 WD 4.00
CFI Plan Fluor 20X MImm (Oil/Glycerin/Water) NA 0.75 WD 0.35-0.33
CFI Plan Apochromat Lambda S 25X Silicone NA 1.05 WD 0.55
CFI Plan Apochromat Lambda S 40X Silicone NA 1.25 WD 0.30
CFI Plan Apochromat VC 60XC Water NA 1.20 WD 0.31-0.28
CFI Apochromat TIRF 60XC Oil NA 1.49 WD 0.13-0.07 (37oC)
CFI Apochromat TIRF 100XC Oil NA 1.49 WD 0.15-0.09 (37oC)
CFI Plan Apo Lambda 60X Oil NA 1.40 WD 0.13
CFI Plan Apo VC 100X Oil NA 1.40 WD 0.13
・Tokai Hit stage top incubator
・Mosaic3 photostimulation unit with 405 nm/450 mW laser

2019