Leica Super-Resolution Microscope TCS SP8 STED

TCS SP8 STED

Leica

・Live-cell confocal imaging
・Gated STED 2D super-resolution imaging with 50-nm (xy) resolutions
・5 laser lines (458, 476, 488, 514, 633 nm) and white light laser (470 – 670 nm)
・1 to 3-color STED imaging by 2 depletion laser lines (592, 660 nm)
・Motorized confocal microscope SP8 (based on Leica DMI 6000 CS)
・Adaptive Focus Control unit (maintains the desired focal plane for long-term imaging)
・Lasers Ar (458, 476, 488, 514 nm), HeNe (633 nm)
White light laser: continuous wave laser in the range of 470-670nm; AOTF allows to choose up to 8 different wavelengths simultaneously; pulsed laser system with a repetition rate of 80 MHz
Continuous wave depletion laser (592, 660 nm) for STED
Ti:Sapphire pulsed laser (Coherent Chameleon)
・Detectors
2ch PMT fluorescence detectors
2ch high-sensitive hybrid GaAsP detector HyD; time gated detection allows improvement of STED lateral (xy) resolution to <50 nm by suppressing short-lived signals at the edge of focal spot
1ch Transmitted light detector
・Scanner
FOV scanner with dual scanning galvanometer mirrors allows for either high speed scanning 7 images/sec at 512 x 512 or high pixel density scanning 8k x 8k (64 megapixels) per channel.
・Motorized XY stage and Z galvo stage for high-precision Z-sectioning
・DIC unit
・Objectives
Dry lens HC PL APO CS 10x/0.40
Oil lens HCX PL APO CS 40x/1.25-0.75, HCX PL APO CS 63x/1.40-0.60, HC PL APO 100x/1.40 STED WHITE
Water lens HCX PL APO 63x/1.20 W CORR CS
・Cell culture chamber with temperature and CO2 Control (Tokai Hit)
・Deconvolution software with STED option (Huygens)

2009